Abstract：Objective：To analyze the main targets and therapeutic mechanisms of Cnidium Monnieri in treating premature ejaculation using network pharmacology and molecular docking technology. Methods： The main active components and their targets of Cnidium Monnieri were obtained from the TCMSP database. Disease-related targets were retrieved from the DrugBank and GeneCards databases. A Cnidium Monnieri-active components-intersecting gene network was constructed. The protein-protein interaction （PPI） network was obtained using the String database. Gene Ontology （GO） analysis and Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway analysis were performed using the DAVID database， followed by molecular docking. Results： A total of 20 active components and 10 key targets of Cnidium Monnieri were identified. GO functional enrichment analysis showed that Cnidium Monnieri mainly regulates functions in the membrane， nucleus， cytoplasm， plasma membrane， transferase， receptor， and kinase. KEGG pathway enrichment analysis indicated that Cnidium Monnieri is involved in the PI3K-Akt signaling pathway， thyroid hormone signaling pathway， cancer pathway， and prostate cancer pathway. Molecular docking results showed stable binding of Cnidium Monnieri's active component （Osthole） to targets such as AKT1，ESR1，EGFR，and JUN. Conclusion： Cnidium Monnieri may exert its therapeutic effects by Osthole on premature ejaculation by acting on targets such as AKT1， ESR1， EGFR， and JUN through the regulation of the PI3K-Akt signaling pathway， thyroid hormone signaling pathway，cancer pathway，and prostate cancer pathway.