Abstract： Objective： To observe the effect of timing auricular point seed-pressing combined with point application on neurological function and sleep quality in patients with insomnia after ischemic stroke. Methods：A total of 90 patients with insomnia after ischemic stroke admitted to Taizhou Central Hospital from December 2019 to October 2023 were selected as the research subjects，and were randomly divided into the control group and the observation group with 45 cases each using a random number table method. The control group was treated with Alprazolam Tablets on the basis of routine treatment， and the observation group was treated with timing auricular point seed-pressing combined with point application on the basis of the control group. Both groups received continuous treatment for four weeks. Compared the clinical effects of two groups， including neurological function indicators [Modified Edinburgh- Scandinavian Stroke Scale （MESSS）]， sleep quality indicators [Pittsburgh Sleep Quality Index （PSQI）， awakening time， sleep latency， sleep efficiency， and number of awakenings]， and laboratory indicators [serum brain-derived neurotrophic factor（ BDNF），melatonin（ MT），serotonin（ 5-HT），norepinephrine（ NE），interleukin-1 β（ IL-1β）， and tumor necrosis factor-α （TNF-α）] levels before and after treatment. Results：After treatment，the total effective rate was 91.11%（ 41/45） in the observation group，which was higher than that of 75.56%（ 34/45） in the control group， the difference being significant （P＜0.05）. After four weeks of treatment，the MESSS scores in both groups were lower than those before treatment and after two weeks of treatment（ P＜0.05），and showed a downward trend（ P＜0.05）；the MESSS scores in the observation group was lower than that in the control group at the same time point （P＜0.05）. After treatment，the PSQI scores in both groups were decreased when compared with those before treatment （P＜0.05），and the score in the observation group was lower than that in the control group （P＜0.05）；the awakening time and sleep latency were both shortened when compared with those before treatment （P＜0.05），and the indexes in the observation group were shorter than those in the control group （P＜0.05）；sleep efficiency was increased when compared with that before treatment （P＜0.05），and the one in the observation group was higher than that in the control group （P＜0.05）； the number of awakenings was decreased when compared with that before treatment （P＜0.05）， and the observation group had fewer awakenings than the control group （P＜0.05）. After treatment，the levels of serum BDNF，MT，and 5-HT in both groups were increased when compared with those before treatment （P＜0.05）， and the levels in the observation group were higher than those in the control group （P＜0.05）；the levels of NE，IL-1 β，and TNF - α in both groups were decreased when compared with those before treatment （P＜0.05），and the levels in the observation group were lower than those in the control group （P＜0.05）. Conclusion： The combination of timing auricular point seed-pressing and point application can effectively improve the neurological function and sleep quality of insomnia patients after ischemic stroke， regulate the levels of sleep related factors and inflammatory factors， and effectively improve clinical effects.