Abstract： Objective： To explore the mechanism of action of Tiaowei Anchang Prescription in the treatment of sepsis using network pharmacology， molecular docking and molecular dynamics simulation. Methods： The potential targets of Tiaowei Anchang Prescription were predicted by TCMSP database. Sepsis-related disease targets were searched using GeneCards， OMlM， TTD and Drugbank. Cytoscape3.9.1 software was used to construct the "drug- component-target" network， and the STRlNG database was used to construct the protein-protein interaction （PPI） network. DAVlD database was used for GO functional enrichment and KEGG pathway richment analysis of genes. AutoDock Vina and PyMOL software were used to dock the active ingredients with the molecules of key targets. Gromacs2022.2 software was used to simulate the molecular dynamics of AKT1 and β-sitosterol. Results： A total of 224 active ingredients and 279 potential targets， 3 718 disease-related targets， and 171 disease-related common targets were obtained through screening， which mainly involved biological processes such as inflammatory response， and the advanced glycation end product （AGE）-receptor for AGE （RAGE） signaling pathway had an impact on sepsis. The results of molecular docking showed that five active ingredients （quercetin， kaempferol， β -sitosterol， naringenin， and isorhamnetin） of Tiaowei Anchang Prescription all had good binding activities with the top 10 key targets （c-Jun protein，p53 protein，interleukin-6，signal transducer and activator of transcription 3，protein kinase B， estrogen receptor α， tumor necrosis factor， NF- κB p65 subunit， mitogen-activated protein kinase 3， and mitogen-activated protein kinase 1） with the degree values of the screened target proteins. Molecular dynamics simulations showed that the binding of AKT1 and β-sitosterol was stable and tight. Conclusion： Tiaowei Anchang Prescription can play a therapeutic role in sepsis through multiple targets and multiple pathways.