Abstract： Objective： To analyze the mechanism of Cuscutae Semen in improving diminished ovarian reserve （DOR） using network pharmacology and molecular docking technology. Methods： The active components of Cuscutae Semen were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform （TCMSP）. Potential targets of the active components and DOR-related targets were predicted through GeneCards，OMIM，and DisGeNET databases. A protein-protein interaction network was constructed using the String database and visualized with Cytoscape software. GO and KEGG enrichment analyses of the intersecting targets were performed using the Hiplot database. Finally，molecular docking verification was conducted using AutoDock software. Results：Cuscutae Semen contains 14 active components including kaempferol，quercetin，and isorhamnetin，with 58 potential targets for DOR regulation，including 10 key targets：AKT1，EGFR，TNF，IL-6，TGFB1，CASP3， MMP9，BCL2，ESR1，and TP53. The core pathways mainly included lipid and atherosclerosis，PI3K-Akt signaling pathways， etc. Molecular docking results showed good binding activity between core components and key targets. Conclusion： The main active components of Cuscutae Semen （kaempferol， quercetin， isorhamnetin， etc.） exert reproductive protective effects by acting on targets such as AKT1， EGFR， TNF， and IL-6 to activate or inhibit signaling pathways including lipid and atherosclerosis，PI3K-Akt，etc.