Abstract： Objective： To observe the clinical efficacy of JIN's three-needle technique combined with transcutaneous electrical acupoint stimulation （TEAS） in the treatment of post-stroke hemiplegia. Methods：A total of 100 patients with post-stroke hemiplegia admitted to Xinchang Hospital Affiliated to Shaoxing University from January 2022 to December 2023 were selected and divided into the combination group and the TEAS group according to the simple randomization method， with 50 cases in each group. The TEAS group received TEAS treatment， and the combination group received JIN's three-needle technique on the basis of the TEAS group. The two groups were all treated for two weeks. The Clinical efficacy， changes in Fugl-Meyer Assessment （FMA） scores， Modified Ashworth Scale （MAS） scores， National Institutes of Health Stroke Scale （NIHSS） scores， Modified Barthel Index （MBI） scores， and second messenger levels [serum cyclic guanosine monophosphate （cGMP）， cyclic adenosine monophosphate （cAMP），and inositol triphosphate （IP3） ] between the two groups were compared before and after treatment. Results：After treatment，the total effective rate in the combination group was 94.00% （47/50）， higher than that of 76.00% （38/50） in the TEAS group （P＜0.05） . The scores of FMA and MBI in the two groups were increased when compared with those before treatment，and the scores of FMA and MBI in the combination group were higher than those in the TEAS group （P＜0.05） . The scores of MAS and NIHSS in the two groups were decreased when compared with those before treatment， and the scores of MAS and NIHSS in the combination group were lower than those in the TEAS group （P＜0.05） . The levels of serum cAMP in the two groups were increased when compared with those before treatment，and the level of serum cAMP in the combination group was higher than that in the TEAS group （P＜0.05） . The levels of serum IP3 and cGMP in the two groups were decreased when compared with those before treatment，and the levels of serum IP3 and cGMP in the combination group were lower than those in the TEAS group （P＜0.05）. Conclusion： JIN's three-needle technique combined with TEAS enhances motor function， neurological recovery，activities of daily living，and overall therapeutic efficacy in patients with post-stroke hemiplegia.