Abstract：Objective：To observe the clinical effect of the combination use of Wandai Decoction and recombinant human interferon on chronic cervicitis complicated with high-risk human papillomavirus（HR-HPV）infection with syndrome of spleen deficiency and prevalence of dampness. Methods： A total of 66 patients with chronic cervicitis complicated with HR-HPV infection with syndrome of spleen deficiency and prevalence of dampness who were treated in the Obstetrics and Gynecology Clinic of Dongguan Traditional Chinese Medicine Hospital from December 2022 to February 2024 were selected and divided into the control group and the observation group according to the random number table method，with 33 cases in each group. The control group was treated with recombinant human interferon， and the observation group was additionally treated with Wandai Decoction based on the treatment in the control group. The clinical effects in the two groups were evaluated， and the traditional Chinese medicine syndrome scores， disappearance time of abnormal vaginal secretions， HPV viral load， levels of inflammatory factors in cervical lavage fluid and adverse reactions were compared between the two groups. Results： The total effective rate was 90.91% （30/33）in the observation group，higher than that of 69.70%（23/33）in the control group（P＜0.05）. After treatment， the traditional Chinese medicine syndrome scores in the two groups were decreased when compared with those before treatment（P＜0.05），and the traditional Chinese medicine syndrome scores in the observation group were lower than those in the control group（P＜0.05）. The disappearance time of abnormal vaginal secretions in the observation group was shorter than that in the control group，the difference being significant（P＜0.05）. After treatment，the HPV viral load in the two groups was reduced when compared with that before treatment（P＜0.05），and the HPV viral load in the observation group was lower than that in the control group（P＜0.05）. After treatment，the levels of interleukin（IL）- 1β，IL-17 and IL-23 in cervical lavage fluid in the two groups were down-regulated when compared with those before treatment（P＜0.05）， and the above three levels in the observation group were lower than those in the control group （P＜0.05）. Conclusion：The combination use of Wandai Decoction and recombinant human interferon can improve the clinical symptoms of patients with chronic cervicitis complicated with HR-HPV infection with syndrome of spleen deficiency and prevalence of dampness， and meanwhile lower the HPV viral load and mitigate the inflammation responses.