基于数据挖掘与网络药理学分析中医药治疗痰瘀互结型非酒精性脂肪肝用药规律
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R285

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广东省中医药局项目(202305132010055580);国家中医药管理局第五批全国中医临床优秀人才研修项目(国中医药办人教函〔2021〕271号)


Analysis of Medication Rules of Traditional Chinese Medicine for Non-Alcoholic Fatty Liver Disease of Phlegm-Stasis Intermingled Type Based on Data Mining and Network Pharmacology
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    目的:基于数据挖掘与网络药理学分析中医药治疗痰瘀互结型非酒精性脂肪肝(NAFLD) 的用药 规律。方法:通过中国知网、中国生物医学文献数据库、维普中文期刊数据库检索自建库至2024年10月发表 的中医药治疗痰瘀互结型NAFLD文献数据。采用Excel 2016、IBM Modeler 18.0软件对药物属性、频次、关联 规则进行统计分析得到关联程度最高药对(山楂-丹参),运用系统药理学方法构建药物-有效成分-靶点网络 及药物-疾病关键靶点的蛋白质相互作用(PPI) 网络图,并运用Metascape数据库了解基因及其相关通路富集 信息;通过综合以上网络分析筛选出关键成分及靶点进行分子对接初步验证。结果:共纳入处方108首,涉及 中药169味。使用频次超过56次为山楂、丹参、泽泻、茯苓;四性分布以微寒、温为主,五味分布以苦、甘、 辛为主;归经频次分布以肝、脾、肺为主,功效分布以利水消肿药、补气药、理气药为主。关联规则分析结果 显示支持度首位为山楂-丹参(70.37%),共筛选出山楂-丹参潜在活性成分65个及其治疗NAFLD的潜在靶点 51个。GO功能分析治疗NAFLD主要有胰岛素的反应、凋亡信号通路的负调控、活性氧的响应等生物过程相 关,KEGG富集涉及癌症通路、脂质和动脉粥样硬化、白细胞介素-17(IL-17) 信号通路、T细胞受体信号等 通路。结论:中药药治疗痰瘀互结型NAFLD,药味以苦、微寒为主,着重调理肝、脾两脏,其中山楂-丹参为 痰瘀互结型NAFLD的核心药物组合。

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    Abstract: Objective: To analyze the medication rules of traditional Chinese medicine for non-alcoholic fatty liver disease(NAFLD)of phlegm-stasis intermingled type using data mining and network pharmacology. Methods: Literature on traditional Chinese medicine treatment of NAFLD of phlegm-stasis intermingled type was retrieved from CNKI, CBM, and VIP databases up to October 2024. Excel 2016 and IBM Modeler 18.0 were used for statistical analysis of herb properties,frequency,and association rules to identify the most strongly correlated herb pair(Crataegi Fructus - Salviae Miltiorrhizae Radix). Systems pharmacology methods were employed to construct herb-active component-target networks and protein-protein interaction(PPI) networks of herb-disease key targets. Metascape database was used for gene and pathway enrichment analysis. Key components and targets were screened for molecular docking validation. Results:108 prescriptions involving 169 herbs were included. High-frequency herbs(>56 uses): Crataegi Fructus, Salviae Miltiorrhizae Radix, Alismatis Rhizoma, and Poria. Herb properties were predominantly slightly cold and warm; flavors mainly bitter, sweet, and acrid. Channel entry mainly distributed on liver, spleen, and lung. Key efficacies were mainly urination promoting and swelling-reducing, qi-tonifying, and qi-regulating herbs. Association rule analysis showed Crataegi Fructus-Salviae Miltiorrhizae Radix had the highest support(70.37%). A total of 65 potential active components and 51 NAFLD-related targets were identified for this pair. GO analysis revealed involvement in insulin response, negative regulation of apoptotic signaling, and reactive oxygen species response. KEGG enrichment highlighted cancer pathways,lipid/atherosclerosis,IL-17 signaling,and T-cell receptor signaling pathways. Conclusion:Traditional Chinese medicine treatment of NAFLD of phlegm-stasis intermingled type primarily uses bitter and slightly cold herbs targeting liver and spleen regulation, with Crataegi Fructus-Salviae Miltiorrhizae Radix as the core herb combination.

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温晓倩,曾江涛,王月英.基于数据挖掘与网络药理学分析中医药治疗痰瘀互结型非酒精性脂肪肝用药规律[J].新中医,2025,57(17):187-193

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