Abstract：Objective：To observe the clinical effect of the combination use of acupotomy lysis at pain points and point application on knee osteoarthritis（KOA）with qi stagnation and blood stasis syndrome. Methods： A total of 86 KOA patients with qi stagnation and blood stasis syndrome treated in The Fourth People's Hospital of Shangqiu from January 2023 to January 2025 were selected and divided into the control group and the observation group by random number table method，with 43 cases in each group. Both groups were treated with conventional western medicine，the control group was additionally treated with acupotomy lysis at pain points，and the observation group was additionally treated with acupoint application based on the treatment in the control group. Both groups were treated for four weeks. The clinical effects and incidence of adverse reactions were compared between the two groups. The changes in traditional Chinese medicine syndrome scores，degree of pain（scores of pain on movement，resting pain and joint tenderness）， inflammatory indicators [tumor necrosis factor-α（TNF-α）， interleukin-6（IL-6）and matrix metalloproteinase-3 （MMP-3）in serum]，and knee joint function [Lysholm Knee Scoring Scale（LKSS），Western Ontario and McMaster Universities Arthritis Index（WOMAC）] were observed before and after treatment in the two groups. Results： After treatment，the total effective rate was 95.34%（41/43）in the observation group，higher than that of 79.07%（34/43）in the control group，difference being significant（P＜0.05）. The traditional Chinese medicine syndrome scores，scores of pain on movement，resting pain，joint tenderness，and WOMAC，and serum levels of TNF-α，IL-6 and MMP-3 in the two groups were decreased when compared with those before treatment. The traditional Chinese medicine syndrome scores，scores of pain on movement，resting pain，joint tenderness，and WOMAC，and serum levels of TNF-α，IL-6 and MMP-3 in the observation group were lower than those in the control group，the differences being significant（P＜ 0.05）. The LKSS scores in the two groups were increased when compared with those before treatment， and the LKSS score in the observation group was higher than that in the control group，differences being significant（P＜0.05）. During the treatment， the incidence of adverse reactions was 2.33%（1/43）in the control group and 6.98%（3/43）in the observation group， there being no significance in the difference（P＞0.05）. Conclusion： The combination use of acupotomy lysis at pain points and point application has a definite curative effect on KOA with qi stagnation and blood stasis syndrome，which can mitigate the pain of patients，decrease the levels of inflammatory indicators and enhance the knee joint function.