Abstract： Objective： To observe the effect of the combination use of hot medicated compress and midnightmidday ebb flow chrono-point application on gastrointestinal dysfunction in sepsis. Methods： A total of 66 patients with gastrointestinal dysfunction in sepsis who were admitted to the intensive care unit of Fangcun Branch of Guangdong Provincial Hospital of Chinese Medicine from April to November 2024 were selected as the observation subjects and divided into the treatment group and the control group according to the random number table method，with 33 cases in each group. The control group was given routine treatment and nursing，and the treatment group was additionally treated with hot medicated compress combined with midnight-midday ebb flow chrono-point application based on the treatment in the control group. Both groups were treated for one week. The gastrointestinal dysfunction scores，acute gastrointestinal injury（AGI）grading，traditional Chinese medicine syndrome scores，related inflammatory indicators，scores of Acute Physiology and Chronic Health Evaluation（APACHE Ⅱ）were compared before and after treatment between the two groups. Results： After treatment， the scores of gastrointestinal dysfunction in the two groups were reduced when compared with those before treatment（P＜0.05），and the above scores in the treatment group were lower than those in the control group（P＜0.05）. After treatment，the AGI grading in the two groups was improved when compared with that before treatment（P＜0.05），and the AGI grading in the treatment group was better than that in the control group（P＜ 0.05）. After treatment， the traditional Chinese medicine syndrome scores in the two groups were decreased when compared with those before treatment（P＜0.05），and the above scores in the treatment group were lower than those in the control group（P＜0.05）. After treatment， the APACHE Ⅱ scores in the two groups were down-regulated when compared with those before treatment（P＜0.05），and the APACHE Ⅱ score in the treatment group was lower than that in the control group（P＜0.05）. After treatment，the levels of C-reactive protein，procalcitonin and neutrophil count in the two groups were reduced when compared with those before treatment（P＜0.05）， and the levels of the three inflammatory indexes in the treatment group were lower than those in the control group（P＜0.05）. Conclusion：The combination use of hot medicated compress and midnight-midday ebb flow chrono-point application can alleviate the manifestation of traditional Chinese medicine syndromes of patients with gastrointestinal dysfunction in sepsis，improve the gastrointestinal function，effectively inhibit the body's inflammatory responses，and reduce the acute gastrointestinal injury.