Abstract：Objective：To analyze the current research status，hotspots，and development trends of defense，qi， nutrient， and blood syndrome differentiation， thus providing references for further research. Methods： Relevant literature on defense，qi，nutrient，and blood syndrome differentiation from 1995 to 2025 was retrieved and screened from the China National Knowledge Infrastructure （CNKI）. Visualization analysis of authors， institutions， and keywords was performed using CNKI and CiteSpace 6.3.R1 software. Results：A total of 739 articles were included， with an overall increasing trend in annual publications. The top four authors in terms of publication volume were AI Bichen，YANG Jin，ZHANG Fuli，and ZHAO Guorong. The institution with the highest publication output was Beijing University of Chinese Medicine. Keyword clustering formed 12 categories， covering basic theory， clinical research， and experimental studies. Research initially focused on theoretical studies in the field of warm disease theory and triple energizer syndrome differentiation， later shifting toward clinical applications， particularly in infectious diseases， dermatological conditions， musculoskeletal and connective tissue disorders， and febrile diseases across various specialties. It also demonstrated significant clinical value in pediatrics and immunology，with current hotspots including epidemic diseases， sepsis， and latent warm disease study. Conclusion： Research on defense， qi， nutrient， and blood syndrome differentiation is in a phase of rapid development， with increasingly refined and enriched theoretical frameworks，as well as advancements in clinical and experimental studies. Diagnostic and therapeutic experiences are being systematically accumulated and summarized. However，its growth rate lags behind the overall progress of traditional Chinese medicine disciplines，necessitating increased research investment. Although substantial theoretical，clinical， and experimental studies have been conducted， comprehensive theoretical discussions remain limited. Future efforts should strengthen the integration of modern scientific research techniques to innovate methodologies and elevate research standards.