Abstract：Objective：To analyze the mechanism of action of Sanren Decoction in treating depression by network pharmacology and molecular docking technology. Methods： The active ingredients and targets of Sanren Decoction were retrieved from the HERB database and BATMAN-TCM database. Disease targets were searched in the GeneCards database，Online Mendelian Inheritance in Man （OMIM） database，and Therapeutic Target Database （TTD）. With the help of the STRING database and Cytoscape 3.9.1 software，the medicine active ingredient-intersection target network was constructed，and protein-protein interaction （PPI） network analysis was conducted. The DAVID database was used to perform Gene Ontology（ GO） functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes（ KEGG） pathway enrichment analysis on the intersection targets. Cytoscape 3.9.1 was applied to screen core targets， and molecular docking verification was carried out for the main active ingredients and core targets. Results： A total of 189 active ingredients of Sanren Decoction were screened out， corresponding to 902 targets. By taking the intersection with 17 988 depression-related disease targets， 146 intersection targets were obtained. The main active ingredients included propapyriogenin A2，cedrol，and myristicin. The core targets included glyceraldehyde-3-phosphate dehydrogenase （GAPDH），AKT serine/threonine-protein kinase （AKT1），tumor necrosis factor （TNF），epidermal growth factor receptor（ EGFR），and catenin beta 1（ CTNNB1）. GO analysis suggested that the intersection targets were mainly involved in biological processes such as protein phosphorylation and phosphorylation. KEGG analysis showed that the relevant signaling pathways mainly included neuroactive ligand-receptor interaction，cancer-related pathways，and cAMP signaling pathway. Molecular docking verification indicated that the binding energies of the main active ingredients to the key targets were all lower than -5 kcal/mol. Conclusion：Sanren Decoction may treat depression by regulating cancer-related pathways，cAMP signaling pathway，and other related pathways through propapyriogenin A2， cedrol，and myristicin acting on targets such as GAPDH，AKT1，TNF，EGFR，and CTNNB1.