Abstract：Objective： To observe the clinical efficacy of Dingchan Anshen Prescription combined with acupoint application in the treatment of Parkinson's disease （PD） complicated by insomnia of liver-kidney yin deficiency type， and its effects on serum levels of amyloid beta 1-42 （Aβ1-42）， phosphorylated Tau-181 （p-Tau181）， and homocysteine （Hcy）. Methods： A total of 136 PD patients with insomnia of liver-kidney yin deficiency type， admitted to the Department of Neurology， Shengzhou Traditional Chinese Medicine Hospital from September 2022 to September 2024，were selected and divided into the control group and the observation group using the random number table method， with 68 cases in each group. Both groups received conventional therapy. The control group received additional acupoint application therapy based on conventional therapy，while the observation group received Dingchan Anshen Prescription in addition to the control group's treatment. Both groups were treated continuously for four weeks. The traditional Chinese medicine syndrome efficacy，traditional Chinese medicine syndrome scores （for tremor of head and body，contracture of the body，restlessness and sleeplessness，difficulty falling asleep），sleep diary data [total sleep time（ TST），sleep efficiency（ SE），sleep onset latency（ SOL）]，Pittsburgh Sleep Quality Index（ PSQI） scores， and serum indicators （Aβ1-42， p-Tau181， Hcy） were compared between the two groups. Adverse reactions were recorded. Results： After four weeks of treatment， the total effective rate of traditional Chinese medicine syndrome efficacy in the observation group was 92.65% （63/68）， which was higher than 79.41% （54/68） in the control group （P＜0.05）. The scores for tremor of head and body， contracture of the body， restlessness and sleeplessness， difficulty falling asleep，and the total traditional Chinese medicine syndrome scores decreased in both groups compared with those before treatment （P＜0.05）；the scores of the four items of traditional Chinese medicine syndrome and the total score in the observation group were lower than those in the control group （P＜0.05）. TST and SE increased，and SOL decreased in both groups compared with those before treatment（ P＜0.05）. The observation group had a longer TST， higher SE， and shorter SOL than the control group （P＜0.05）. The scores for sleep quality， sleep latency， sleep duration， habitual sleep efficiency， sleep disturbances， use of sleeping medication， daytime dysfunction， and the total PSQI score decreased in both groups compared with those before treatment （P＜0.05）；the scores for these seven dimensions of PSQI and the total score in the observation group were lower than those in the control group （P＜0.05）. Serum Aβ1-42 levels in both groups increased compared with those before treatment （P＜0.05）， while serum p- Tau181 and Hcy levels in both groups decreased compared with those before treatment （P＜0.05）. The serum Aβ1-42 level in the observation group was higher than that in the control group （P＜0.05），and the levels of serum p-Tau181 and Hcy in the observation group were lower than those in the control group（P＜0.05）. No adverse reactions occurred in either group during the treatment. Conclusion： Dingchan Anshen Prescription combined with acupoint application is effective in treating PD complicated by insomnia of liver-kidney yin deficiency type. It can alleviate symptoms，improve sleep quality，increase serum Aβ1-42 levels，and decrease p-Tau181 and Hcy levels.