Abstract：Objective：This study observed the effects of matrine combined with Jujubae Fructus and Glycyrrhizae Radix et Rhizoma Praeparata cum Melle in treating heart failure patients with reduced ejection fraction of qi deficiency， blood stasis and water retention type， comparing changes in matrix metalloproteinases （MMPs）/tissue inhibitor of metalloproteinase-1 （TIMP-1） and heart function-related indicators before and after treatment. Methods： A total of 72 cases of HF patients admitted to Zhanjiang First Hospital of Traditional Chinese Medicine from November 2022 to December 2024 were selected and divided into the control group and the trial group according to the random number table method，with 36 cases in each group. Due to case dropouts，the final numbers were 33 cases in the trial group and 34 cases in the control group. The control group received standard heart failure drug treatment， while the trial group received additional intervention with matrine combined with Jujubae Fructus and Glycyrrhizae Radix et Rhizoma Praeparata cum Melle on top of standard treatment. After eight weeks of treatment，the levels of matrix metalloproteinase- 1 （MMP-1）， matrix metalloproteinase-9 （MMP-9）， TIMP-1， N-terminal pro-brain natriuretic peptide （NTproBNP）， left ventricular end-diastolic diameter （LVEDD）， left ventricular end-systolic diameter （LVESD）， left ventricular ejection fraction （LVEF）， and traditional Chinese medicine syndrome scores were compared between the two groups before and after treatment. Results： After treatment， MMP-1 levels in the two groups decreased when compared with those before treatment （P＜0.05）， and the level in the trial group was lower than that in the control group （P＜0.05）； the difference in MMP-1 changes before and after treatment between the two groups was significant （P＜0.05）. By paired rank-sum test， the difference in MMP-1 levels in the trial group before and after treatment was significant （P＜0.05），as was the difference in the control group （P＜0.05）. After treatment，MMP-9 levels decreased in the two groups compared to before treatment （P＜0.05）， with the trial group showing lower level than the control group （P＜0.05）；the difference in MMP-9 changes before and after treatment between the two groups was significant （P＜0.05）. By paired rank-sum test，the difference in MMP-9 levels in the trial group before and after treatment was significant （P＜0.05），as was the difference in the control group （P＜0.05）. After treatment，TIMP-1 levels increased in the two groups compared to before treatment （P＜0.05），with the trial group showing higher level than the control group （P＜0.05）；the difference in TIMP-1 changes before and after treatment between the two groups was significant （P＜0.05）. By paired rank-sum test，the difference in TIMP-1 levels in the trial group before and after treatment was significant （P＜0.05）， as was the difference in the control group （P＜0.05）. After treatment， NTproBNP levels decreased in the two groups compared to before treatment （P＜0.05），with the trial group showing lower level than the control group （P＜0.05）；the difference in NT-proBNP changes before and after treatment between the two groups was significant （P＜0.05）. By paired rank-sum test，the difference in NT-proBNP levels in the trial group before and after treatment was significant （P＜0.05），as was the difference in the control group （P＜0.05）. By paired rank-sum test，the difference in LVEDD in the trial group before and after treatment was significant （P＜0.05），as was the difference in the control group （P＜0.05）. By paired rank-sum test， the difference in LVESD in the trial group before and after treatment was significant （P＜0.05）， as was the difference in the control group （P＜0.05）. After treatment， LVEF increased in the two groups compared to before treatment （P＜0.05）， with the trial group showing higher LVEF than the control group （P＜0.05）；the difference in LVEF changes before and after treatment between the two groups was significant （P＜0.05）. By paired rank-sum test，the difference in LVEF in the trial group before and after treatment was significant （P＜0.05）， as was the difference in the control group （P＜0.05）. After treatment， traditional Chinese medicine syndrome scores decreased in the two groups compared to before treatment （P＜0.05）， with the trial group showing lower score than the control group （P＜0.05）； the difference in traditional Chinese medicine syndrome score changes before and after treatment between the two groups was significant （P＜0.05）. By paired rank-sum test，the difference in traditional Chinese medicine syndrome scores in the trial group before and after treatment was significant （P＜0.05）， as was the difference in the control group （P＜0.05）. Conclusion： Matrine combined with Jujubae Fructus and Glycyrrhizae Radix et Rhizoma Praeparata cum Melle in treating heart failure patients with reduced ejection fraction of qi deficiency， blood stasis and water retention type can inhibit ventricular remodeling and improve heart function by regulating MMPs/TIMP imbalance.