Abstract：Objective：To observe the clinical effect of Ziyin Jieyu Prescription on Parkinson's disease （PD） with depression of yin deficiency and liver stagnation type. Methods： A total of 108 PD patients with depression of yin deficiency and liver stagnation type admitted to Wenzhou TCM Hospital of Zhejiang Chinese Medical University from January to December 2024 were divided into the combination group and the western medicine group according to the random number table method， with 54 cases in each group. The western medicine group was treated with antiparkinsonian medications and Fluoxetine Hydrochloride Capsules，and the combination group received Ziyin Jieyu Prescription. Both groups were treated for eight weeks. Before and after treatment， the Hamilton Depression Scale （HAMD） scores， the Geriatric Depression Scale-15 （GDS-15） scores， the traditional Chinese medicine syndrome scores，the third part of the Unified Parkinson Disease Rating Scale （UPDRS-Ⅲ） scores，and the Pittsburgh Sleep Quality Index （PSQI） scores were evaluated. The levels of neurotransmitters [5- hydroxytryptamine （5-HT）， norepinephrine （NE）， dopamine （DA）] were detected. The clinical efficacy and incidence of adverse reactions were compared between the two groups. Results： After treatment， the total effective rate in the combination group was higher than that in the western medicine group （P＜0.05）. After treatment，HAMD scores， GDS-15 scores， traditional Chinese medicine syndrome scores， UPDRS-Ⅲ scores， and PSQI scores were decreased when compared with those before treatment （P＜0.05）；the above five scores in the combination group were lower than those in the western medicine group （P＜0.05）. After treatment，the levels of 5-HT，NE，and DA in the two groups were increased when compared with those before treatment （P＜0.05）； the above three levels in the combination group were higher than those in the western medicine group （P＜0.05）. There was no difference being found in the incidence of adverse reactions between the two groups （P＞0.05）. Conclusion： Ziyin Jieyu Prescription combined with western medicine has a good effect on PD with depression of yin deficiency and liver stagnation type， which can effectively reduce depressive symptoms， mitigate traditional Chinese medicine syndromes related to yin deficiency and liver stagnation，and improve sleep quality and motor function with good safety.