Abstract： With the continuous development of microecological research， the regulation of gut microbiota has become an important approach to improving health status. Eliminating pathogens and reinforcing healthy qi is a fundamental therapeutic principle in traditional Chinese medicine （TCM）. Among the healthy qi-reinforcing herbs， yin-nourishing herbs are widely used in clinical practice to treat various diseases such as diabetes，palpitations，yin deficiency-induced exogenous fever， and constipation. Modern pharmacological and clinical studies have shown that polysaccharides contained in yin-nourishing herbs serve as important sources for maintaining the structure and function of gut microbiota， exhibiting multiple effects including immunomodulation， anti-inflammation， anti-tumor activity， and improvement of glucose and lipid metabolism disorders. This review summarizes research on how polysaccharide components from yin-nourishing herbs regulate gut microbiota homeostasis in vivo and in vitro，promote the production of metabolites such as short-chain fatty acids （SCFAs），and modulate signaling pathways. It also analyzes the potential of these polysaccharides as prebiotics combined with probiotics， providing a scientific basis for developing polysaccharide components from yin-nourishing herbs into prebiotics.