Abstract：Objective：To observe the clinical efficacy of Xingnao Kaiqiao acupuncture combined with electronic biofeedback in the treatment of post-stroke upper limb dysfunction. Methods： A total of 100 post-stroke hemiplegia patients admitted to the Rehabilitation Department of Chinese Medicine Hospital of Puyang from September 2024 to August 2025 were selected and randomly divided into the control group and the observation group using the random number table method， with 50 cases in each group. Both groups received basic treatment. The control group received electronic biofeedback therapy，while the observation group received additional Xingnao Kaiqiao acupuncture based on the control group's treatment. Clinical efficacy was compared between the two groups. The following were compared before and after treatment between the two groups： Brunnstrom stages of the upper limb， active range of motion （AROM） for wrist dorsiflexion and shoulder flexion， Upper Extremity Function Test（UEFT） score， Fugl- Meyer Assessment for Upper Extremity（FMA-UE） score， Modified Barthel Index（MBI） score， root mean square （RMS） value of surface electromyography for the forearm extensor muscles， triceps brachii， and deltoid muscles， as well as serum levels of central nervous system-specific protein β（S100β） ， neuron-specific enolase （NSE）， nerve growth factor （NGF）， and brain-derived neurotrophic factor （BDNF）. Results： The total effective rate was 96.00% （48/50） in the observation group and 84.00% （42/50） in the control group， with a statistically significant difference between the two groups （P＜0.05）. After treatment， Brunnstrom stages improved in both groups compared to before treatment （P＜0.05），and the observation group showed superior Brunnstrom stages to the control group （P＜0.05）. After treatment， AROM for wrist dorsiflexion and shoulder flexion increased in both groups compared to before treatment （P＜0.05）， and the AROM for wrist dorsiflexion and shoulder flexion in the observation group was greater than that in the control group （P＜0.05）. After treatment，MBI，FMA-UE，and UEFT scores increased in both groups compared to before treatment （P＜0.05），and MBI，FMA-UE，and UEFT scores in the observation group were higher than those in the control group （P＜0.05）. After treatment， the RMS values of the forearm extensor muscles， triceps brachii， and deltoid muscle increased in both groups compared to before treatment （P＜0.05），and all three values were greater in the observation group than in the control group （P＜0.05）. After treatment， serum levels of S100β and NSE decreased in both groups compared to before treatment （P＜0.05）， and the leveles of both indicators in the observation group were lower than those in the control group （P＜0.05）. Serum levels of NGF and BDNF increased in both groups compared to before treatment （P＜0.05）， and the levels of both indicators in the observation group were higher than those in the control group（P＜0.05）. Conclusion： Xingnao Kaiqiao acupuncture combined with electronic biofeedback can regulate levels of inflammatory factors and neurotrophic factors，enhance limb motor function and daily living ability，thereby improving clinical efficacy in patients with poststroke upper limb dysfunction.