Abstract：Objective：To investigate the differences in meridian characteristics among various traditional Chinese medicine （TCM） constitution types in patients with chronic fatigue syndrome （CFS）. Methods： A retrospective analysis was conducted on 231 patients diagnosed with CFS at the Preventive Treatment Center of Foshan Hospital of Traditional Chinese Medicine from September 2023 to September 2024. All patients underwent TCM constitution identification and meridian detection， and were grouped according to their TCM constitution types. Differences in meridian parameters were compared between patients with balanced constitution and those with other TCM constitution types. Results：The predominant constitution types in the CFS patients were yang-deficiency （51 cases，22.1%），qideficiency （45 cases，19.5%），and damp-heat （45 cases，19.5%）. Statistically significant differences were observed in autonomic nerve values and upper-lower balance values among CFS patients with various TCM constitution types （P＜ 0.05）. No statistically significant differences were found in yin-yang balance values， left-right balance values， or conduction coefficient values among CFS patients with various TCM constitution types （P＞0.05）. Compared with CFS patients with balanced constitution， those with qi-deficiency， yang-deficiency， yin-deficiency， phlegm-damp， damp-heat，and inherited special constitutions showed no statistically significant differences in autonomic nerve values or upper-lower balance values （P＞0.05）. However，compared with balanced-constitution CFS patients，patients with blood-stasis constitution showed higher autonomic nerve values， and those with qi-stagnation constitution exhibited higher upper-lower balance values （P＜0.05）. Compared with balanced-constitution CFS patients，meridian detection values were significantly lower in the right large intestine meridian and right spleen meridian of qi-deficiency constitution CFS patients； in the bilateral kidney meridians and left pericardium meridian of yang-deficiency constitution CFS patients；in the left pericardium meridian and right liver meridian of yin-deficiency constitution CFS patients；in the left pericardium meridian of damp-heat constitution CFS patients；and in the right spleen meridian of phlegm-damp constitution CFS patients （P＜0.05）. In contrast，meridian detection values were significantly higher in the left stomach meridian of phlegm-damp constitution CFS patients （P＜0.05）. Conclusion：Yang-deficiency is the most common TCM constitution type in CFS patients. Different TCM constitutions exhibit distinct meridian characteristics and present specific patterns of left-right imbalance. Among these， meridian imbalances in the liver， spleen， and kidney meridians are closely associated with qi-deficiency， yang-deficiency， yin-deficiency， and phlegm-damp constitutions.