Abstract： This article introduces Professor YUAN Qing′s clinical experience in preventing and treating chemotherapy-induced nausea and vomiting with spirit-regulating acupuncture based on the brain-gut axis theory. The brain-gut axis is a bidirectional neuro-endocrine communication network between the brain and the gut， and its dysfunction is an important underlying mechanism of chemotherapy-induced nausea and vomiting. Grounded in this theory and clinical practice， Professor YUAN holds that the essential pathogenesis of chemotherapy-induced nausea and vomiting is "direct impairment of the middle earth by chemotoxic agents， leading to dysfunction of the pivot mechanism，together with disturbance of the heart-mind triggering reversed stomach qi." The core therapeutic principle is therefore defined as "regulating the mind and harmonizing the stomach. " An integrated acupuncture protocol for prevention and treatment is established： "fortify healthy qi" before chemotherapy， "regulate stomach qi" during chemotherapy， and "benefit original qi" after chemotherapy. By needling cranial points to modulate central neural activity and spleen-stomach points to adjust intestinal function，the brain and gut are regulated concomitantly，which effectively alleviates chemotherapy-induced nausea and vomiting.