Abstract： Objective： To analyze the distribution characteristics of traditional Chinese medicine constitution， and their correlation with human papillomavirus （HPV） infection，cervical cytology results in elderly women undergoing physical examinations， and to provide theoretical basis for traditional Chinese medicine constitution conditioning， prevention and treatment of HPV infection， and cervical lesions. Methods： The clinical data of elderly women who underwent the first physical examination in the Physical Examination Center of Shenzhen Hospital （Futian） of Guangzhou University of Chinese Medicine from June 1， 2022 to June 30， 2024 were retrospectively analyzed. The infection rate and genotype distribution of HPV，the detection rate and distribution characteristics of cervical cytological abnormalities， the distribution characteristics of traditional Chinese medicine constitution and their correlation with HPV infection were analyzed. Results： The total positive rate of HPV was 24.04% （113/470） in 470 elderly women undergoing physical examinations，of which the positive rate of high risk （HR）-HPV was 17.02% （80/470）. The most common HR-HPV genotypes were HPV52 and HPV58. The detection rate of abnormal cervical cytology was 26.55%（ 30/113） in the HPV positive group，and that was 7.69%（ 27/351） in the HPV negative group，the difference being significant （χ2=28.207， P＜0.001）. Among the 404 women who filled in the Constitution in Chinese Medicine Questionnaire， 122 （30.20%） had a single constitution， and 282 （69.80%） had a combined constitution. The most common constitutions were qi-deficiency constitution， and dampness-heat constitution. Yin-deficiency constitution， dampness-heat constitution，qi stagnation constitution and phlegm-dampness constitution were statistically significant between the HPV positive group and the HPV negative group （P＜0.05），among which yin deficiency，qi stagnation， and phlegm-dampness were high risk constitutions of HPV infection. Conclusion：The detection rate of HPV infection and abnormal cervical cytology in elderly women is high， and regular cervical cancer screening is still needed. HPV infection is related to traditional Chinese medicine constitution. Yin-deficiency constitution，qi stagnation constitution， and phlegm-dampness constitution are risk factors for HPV infection.