Abstract： Objective： To observe the effects of different sequential timing of scalp acupuncture combined with walking-assisted functional electrical stimulation （FES） on walking function in patients with post-stroke hemiplegia. Methods：A total of 102 stroke patients were enrolled. They were hospitalized at Xiangyang Central Hospital Affiliated to Hubei University of Arts and Science， from August 2023 to April 2024， met the inclusion criteria and voluntarily participated in this study. They were randomly divided into three groups， with 95 patients ultimately completing the study. In the scalp acupuncture-priority group （31 cases），patients received 30 minutes of scalp acupuncture followed by 30 minutes of walking-assisted FES. In the synchronous treatment group （32 cases）， patients received scalp acupuncture and walking-assisted FES concurrently for 30 minutes per session. In the scalp acupuncture-delayed group （32 cases），patients received 30 minutes of walking-assisted FES followed by 30 minutes of scalp acupuncture. All treatments were administered once daily， six times per week， for two weeks. Brunnstrom staging， the 10-meter walk test （10MWT）， and Walkerview center-of-gravity range of motion assessment were evaluated before treatment and after two weeks of treatment （12 sessions）. Results：After two weeks of treatment：①Brunnstrom staging results showed that the Brunnstrom stages in all three groups were significantly improved compared to before treatment （P＜ 0.05）， and the Brunnstrom stages in the scalp acupuncture-priority group and the scalp acupuncture-delayed group were both significantly higher than that in the synchronous treatment group （P＜0.05）. ② The 10MWT results showed that the average walking speed in all three groups was significantly increased compared to before treatment （P＜0.05）， and the speed in the scalp acupuncture-priority group was significantly higher than that in the synchronous treatment group （P＜0.05）. ③ The Walkerview center-of-gravity range of motion results showed that the range of motion in all three groups was significantly reduced compared to before treatment （P＜0.05），and the range of motion in the scalp acupuncture-priority group was significantly lower than that in the synchronous treatment group （P＜0.05）. Conclusion： Stroke inpatients receiving two weeks of sequential timing treatment with scalp acupuncture combined with walking-assisted FES showed significant improvement in walking function. Notably， the sequential protocols of both the scalp acupuncture-priority group and the scalp acupuncture-delayed group yielded more significant effects compared to other regimens.